Dietmar J. Manstein, Publications

Journal Articles

• Fedorov, R., Böhl, M., Tsiavaliaris, G., Hartmann, F. K., Taft, M. H., Baruch, P., Brenner, B., Martin, R., Knölker, H.-J., Gutzeit, H. O., and Manstein, D. J. (2009).
  Allosteric inhibition of myosin motor activity by pentabromopseudilin.
  Nat. Struct. Mol. Biol., doi:10.1038/nsmb.1542 (pdf, 1 MB) E-Print (pdf, 2,5 MB)
• Tsiavaliaris, G., Fujita-Becker, S., Dürrwang, U., Diensthuber, R. P., Geeves, M. A., and Manstein, D. J. (2008).
  Mechanism, Regulation, and Functional Properties of Dictyostelium Myosin-1B.
  J Biol Chem 283, 4520-4527. (Abstract)
• Taft, M. H., Hartmann, F. K., Rump, A., Keller, H., Chizhov, I., Manstein, D. J., and Tsiavaliaris, G. (2008).
  Dictyostelium Myosin-5b is a conditional processive motor.
  J Biol. Chem 283, 26902-26910. (Abstract)
• Stolboushkina, E., Nikonov, S., Nikulin, A., Blasi, U., Manstein, D. J., Fedorov, R., Garber, M., and Nikonov, O. (2008).
  Crystal structure of the intact archaeal translation initiation factor 2 demonstrates very high conformational flexibility in the alpha- and beta-subunits.
  J Mol. Biol. 382, 680-691. (Abstract)
• Nikonov, O., Stolboushkina, E., Nikulin, A., Hasenohrl, D., Blasi, U., Manstein, D. J., Fedorov, R., Garber, M., and Nikonov, S. (2007).
  New insights into the interactions of the translation initiation factor 2 from archaea with guanine nucleotides and initiator tRNA.
  J Mol Biol 373, 328-336. (Abstract)
• Hachikubo, Y., Ito, K., Schiefelbein, J., Manstein, D. J., and Yamamoto, K. (2007).
  Enzymatic activity and motility of recombinant Arabidopsis myosin XI. MYA1
  Plant Cell Physiol 48, 886-891. (Abstract)
• Fujita-Becker, S.,Tsiavaliaris, G., Ohkura, R., Shimada, T., Manstein, D.J., and Sutoh, K. (2006).
  Functional characterization of the N-terminal region of myosin-2.
  J Biol Chem 281, 36102-36109. (Abstract)(pdf)
• Fedorov, R., Witte, G., Urbanke, C., Manstein, D.J., and Curth, U. (2006).
  3D structure of Thermus aquaticus single-stranded DNA-binding protein gives insight into the functioning of SSB proteins.
  Nucleic Acids Res 34, 6708-6717. (Abstract)
• Manstein, D.J. (2006).
  Motorproteine: in vitro Motilitätsversuche.
  Biospektrum 12, 632-634.(pdf)
• Ulrike Dürrwang, Setsuko Fujita-Becker, Muriel Erent, F. Jon Kull, Georgios Tsiavaliaris, Michael A. Geeves, and Dietmar J. Manstein (2006)
  “Dictyostelium myosin-IE is a fast molecular motor involved in phagocytosis”
  J. Cell Science 119, 550-558 (abstract) (pdf)
• Reubold, T. F., Eschenburg, S., Becker, A., Leonard, M., Schmid, S. L., Vallee, R. B., Kull, F. J. and Manstein, D. J. (2005)
  “Crystal structure of the GTPase domain of rat dynamin 1”
  Proc. Natl. Acad. Sci. U.S.A. 102, 13093-13098 (pdf)
• Geeves, M. A., Fedorov, R. and Manstein, D. J. (2005)
  “Molecular mechanism of actomyosin-based motility.”
  Cell Mol. Life Sci. 62, 1462-1477 (pdf)
• Fujita-Becker, S., Dürrwang, U., Erent, M., Clark, R. J., Geeves, M. A. and Manstein, D. J. (2005)
  “Changes in Mg2+ ion concentration and heavy chain phosphorylation regulate the motor activity of a class I myosin”
  J. Biol. Chem. 280, 6064-6071 (pdf)
• Van Dijk, J., Lafont, C., Knetsch, M. L., Derancourt, J., Manstein, D. J., Long, E. C. and Chaussepied, P. (2004)
  “Conformational changes in actin-myosin isoforms probed by Ni(II).Gly-Gly-His reactivity”
  J. Muscle Res. Cell Motil. 25, 527-537
• Manstein, D. J. (2004)
  “Molecular engineering of myosin”
  Philos. Trans. R. Soc. Lond. B Biol. Sci. 359, 1907-1912 (pdf)
• Dijk, J. V., Lafont, C., Knetsch, M. L., Derancourt, J., Manstein, D. J., Long, E. C. and Chaussepied, P. (2004)
  “Conformational changes in actin-myosin isoforms probed by Ni(II).Gly-Gly-His reactivity”
  J. Muscle Res. Cell Motil. 25, 527-537
• Tsiavaliaris, G., Fujita-Becker, S. & Manstein, D.J. (2004)
  “Molecular engineering of a backwards moving myosin motor”
  Nature 427, 558-561 (Abstract)(pdf)
• Ito K., Kashiyama T., Shimada K, Yamaguchi A., Awata J, Hachikubo Y., Manstein D.J., Yamamoto K. (2003)
  “Recombinant motor domain constructs of Chara corallina myosin display fast motility and high ATPase activity”
  Biochem. Biophys. Res. Commun. 312, 958-64. (Abstract) (pdf)
• Krylyshkina, O., Anderson, K.I., Kaverina, I., Upmann, I., Manstein, D.J., Small, J.V. & Toomre, D.K.
  “Nanometer targeting of microtubules to focal adhesions”
  J. Cell Biol., 161, 853-859. (Abstract)(pdf)
• Reubold, T.F., Eschenburg, S., Becker, A., Kull, F.J. & Manstein, D.J. (2003)
  “A structural model for actin-induced nucleotide release in myosin”
  Nature Struct. Biol., 10, 826-830. (Abstract)(pdf)
• Tsiavaliaris, G. & Manstein, D.J. (2003)
  “TIRF-Mikroskopie und ihre Anwendung in der Biologie”
  Biospektrum, 596-600. (pdf)
• Schlosser, A., Klockow, B., Manstein, D.J., & Lehmann, W.D. (2003)
  “Analysis of post-translational modification and characterization of the domain structure of dynamin A from Dictyostelium discoideum.”
  J. Mass. Spectrom. 38 (3) 277-282 (Abstract)(pdf)
• Ballweber, E., Kiessling, P., Manstein, D.J., & Mannherz, H.G. (2003)
  “Interaction of Myosin subfragment 1 with forms of monomeric actin”
  Biochemistry 42 (10) 3060-3069 (Abstract)
• Knetsch, M.L.W., Tsiavaliaris, G., Zimmermann, S., Rühl, S., &. Manstein, D.J. (2002)
  “Expression vectors for studying cytoskeletal proteins in Dictyostelium discoideum”
  J. Mus. Res. Cell Motil.23, 605-611 (Abstract)
• Tsiavaliaris, G., Fujita-Becker, S., Batra, R., Levitsky, D.I., Kull, F.J., Geeves, M.A. & Manstein, D.J. (2002)
  “Mutations in the relay loop region result in dominant-negative inhibition of myosin II function in Dictyostelium”
  EMBO Reports, 3, 1099-1105 (Abstract) (pdf)
• Linke, W.A., Kulke, M., Li, H., Fujita-Becker S., Neagoe C., Manstein, D.J., Gautel, M., Fernandez, J.M. (2002)
  “PEVK Domain of Titin: An Entropic Spring with Actin-Binding Properties”.
  J. Struct. Biol. 137, 194-205. (Abstract)
• Kollmar, M. Dürrwang, U., Kliche, W., Manstein, D.J., & Kull, F.J. (2002)
  “Crystal structure of the motor domain of a class-I myosin”.
  EMBO J. 21, 2517-25 (Abstract)
• Herm, A., Weiss, S., Fujita-Becker, S., Ruff, C., Meyhöfer, E., Soldati, T., Manstein, D.J., Geeves, M.A., & Soldati, D. (2002)
  “Toxoplasma gondii class XIV myosin is a fast, single-headed, plus-end directed motor”.
  EMBO J. 21, 2149-2158. (Abstract) (pdf, 400 kB) -
• Klockow, B., Tichelaar, W., Madden, D., Niemann, H.H., Akiba, T., Hirose, K., & Manstein, D.J. (2002)
  “The dynamin a ring complex: molecular organization and nucleotide-dependent conformational changes”.
  EMBO J. 21, 240-250. (Abstract) (pdf, 500 kB)
• Niemann, H.H., Knetsch, M.L.W. Scherer, A., Manstein, D.J., & Kull, F.J. (2001)
  “Crystal structure of a dynamin GTPase domain”
  EMBO J., 21, 5813-5821. (Abstract) (pdf, 400 kB)
• Kulke, M., Fujita-Becker, S., Rostkova, E., Neagoe, C., Labeit, D., Manstein, D.J., Gautel, M., & Linke, W.A. (2001)
  “Interaction between PEVK-titin and actin filaments: origin of a viscous force component in cardiac myofibrils”.
  Circ. Res., 89, 874-881. (Abstract)
• Toomre, D. & Manstein, D.J. (2001)
  “Lighting up the cell surface with evanescent wave microscopy”
  Trends Cell Biol., 11, 298-303. (Abstract) (pdf, 2.5 MB)
• Knetsch, M.L.W., Schäfers, N., Horstmann, H. & Manstein, D. J. (2001)
  “A Bcr/Abr-related protein controls the organization of a tubolocisternal organelle, F-Actin dynamics, and cytokinesis in Dictyostelium”
  EMBO J., 20, 1620-1629. (Abstract) (pdf, 350 kB)
• Ruff, C., Furch, M., Brenner, B., Manstein, D.J. & Meyhöfer, E. (2001)
  “Single-molecule tracking of myosins with genetically engineered amplifier domains”
  Nature Struct. Biol., 8, 226-229. (Abstract) (pdf, 590 kB)
• Kliche, W., Fujita-Becker, S., Kollmar, M., Manstein, D.J. & Kull, F.J. (2001)
  “Structure of a genetically engineered molecular motor”
  EMBO J., 20, 40-46. (Abstract)
• Furch, M., Remmel, B., Geeves, M.A. & Manstein, D.J. (2000)
  “Stabilization of the actomyosin complex by negative charges on myosin”
  Biochemistry, 39, 11602-11608. (Abstract) (pdf)
• Ponomarev, M.A., Furch, M., Levitsky, D.I. & Manstein, D.J. (2000)
  “Charge changes in loop 2 affect the thermal unfolding of the myosin motor domain bound to F-actin”
  Biochemistry, 39, 4527-4532. (Abstract) (pdf)
• Van Dijk, J., Furch, M., Lafont, C., Manstein, D.J., & Chaussepied, P. (1999)
  “Functional characterization of the secondary actin binding site of myosin II”
  Biochemistry, 38, 15078-15085. (Abstract) (pdf, 160 kB)
• Batra, R. & Manstein, D.J. (1999)
  “Functional characterisation of Dictyostelium myosin II with conserved tryptophanyl residue 501 mutated to tyrosine”
  Biol. Chem. 380, 1017-1023. (Abstract)
• Furch, M., Fujita-Becker, S., Geeves, M.A., Holmes, K.C. & Manstein, D.J. (1999)
  “Role of the salt-bridge between switch-1 and switch-2 of Dictyostelium myosin”
  J. Mol. Biol. 290, 797-809. (Abstract) (pdf, 330 kB)
• Knetsch, M.L.W., Uyeda, T.Q.P., & Manstein, D.J. (1999)
  “Disturbed communication between actin- and nucleotide binding sites in a myosin II with truncated 50K/20K junction”.
  J. Biol. Chem. 274, 20133-20138. (Abstract) (pdf, 122 kB)
• Batra, R., Geeves, M.A., &. Manstein, D.J. (1999)
  “Kinetic analysis of myosin motor domains with glycine-to-alanine mutations in the reactive thiol region”
  Biochemistry 38, 6126-6134 (Abstract) (pdf)
• Van Dijk, J., Furch, M., Derancourt, J., Batra, R., Knetsch, M.L.W., Manstein, D.J., & Chaussepied, P.(1999)
  “Differences in the interaction of actin with the motor domains of non-muscle and muscle myosin II”
  Europ. J. Biochem. 260, 672-683. (Abstract) (pdf, 844 kB)
• Wienke, D.C., Knetsch, M.L.W., Neuhaus, E.M., Reedy, M.C. & Manstein, D.J. (1999)
  “Disruption of a dynamin homologue affects en­docytosis, organelle morphology and cytokinesis in Dictyostelium discoideum”
  Mol. Biol. Cell 10, 225-243. (Abstract) (pdf, 966 kB)
• Friedman, A. L., Geeves, M. A., Manstein, D.J. & Spudich, J.A. (1998)
  “Kinetic Characterization of myosin head fragments with long-lived myosin.ATP states”
  Biochemistry 37, 9679-9687. (Abstract) (pdf)
• Furch, M., Geeves, M.A. & Manstein, D.J. (1998)
  “Modulation of actin affinity and actomyosin ATPase by charge changes in the myosin motor domain”
  Biochemistry 37, 6317-6326. (Abstract) (pdf)
• Levitsky, D.I., Ponomarev, M.A., Geeves, M.A., Shnyrov, V.L. & Manstein, D.J. (1998)
  “Differential scanning calorimetric study of the thermal unfolding of the motor domain fragments of Dictyostelium discoideum myosin II”
  Europ. J. Biochem. 251, 275-280. (Abstract) (pdf, 11 MB)
• Kurzawa, S.E., Manstein, D.J. & Geeves, M.A. (1997)
  “Dictyostelium discoideum myosin II: characterization of functional motor fragments”
  Biochemistry 36, 317-323. (Abstract) (pdf, 163 kB)
• Anson, M. Geeves, M.A., Kurzawa, S. & Manstein, D.J. (1996)
  “Myosins with artificial lever arms”
  EMBO J. 15, 6069-6074. (Abstract) (pdf, 15 MB)
• Woodward, S.K.A., Geeves, M.A. & Manstein, D.J. (1995)
  “Kinetic characterization of the catalytic domain of Dictyostelium discoideum myosin”
  Biochemistry 34, 16056-16064. (Abstract) (pdf, 15 MB)
• Manstein, D.J., Schuster, H.-P., Morandini, P. & Hunt, D.M. (1995)
  “Cloning vectors for the production of proteins in Dictyostelium discoideum”
  Gene 162, 129-134. (Abstract) (pdf, 436 kB)
• Manstein, D.J. & Hunt, D.M. (1995)
  “Overexpression of myosin motor domains in Dictyostelium discoideum: Screening of transformants and pu­rification of the affinity tagged protein”
  J. Mus. Res. Cell Motil. 16, 325-332. (Abstract) (pdf, 13 MB)
• Schröder, R.R., Manstein, D.J., Jahn, W., Holden, H.M., Rayment, I., Holmes, K.C. & Spudich, J.A. (1993)
  “Three-dimensional atomic model of F-actin decorated with Dictyostelium myosin S1”
  Nature 364, 171-174. (Abstract) (pdf, 4.9 MB)
• Ritchie, M.D., Geeves, M.A., Woodward, S.K.A. & Manstein, D.J. (1993)
  “Kinetic characterization of a cytoplasmic motor domain expressed in Dictyostelium discoideum”
  Proc. Natl. Acad. Sci. USA 90, 8619-8623. (Abstract) (pdf)
• Manstein, D.J., Ruppel, K.M., Kubalek, L. & Spudich, J.A. (1991)
  “Manipulation and expression of molecular motors in Dictyostelium discoideum”
  J. Cell Science, 14s, 63-65. (Abstract)
• Egelhoff, T.T., Titus, M.A., Manstein, D.J., Ruppel, K.M. & Spudich, J.A. (1991)
  “Molecular genetic tools for the study of the cytoskeleton in Dictyostelium”
  Meth. Enzymol. 196, 319-334. (Abstract) (pdf, 14 MB)
• Egelhoff, T.T., Manstein, D.J. & Spudich, J.A. (1990)
  “Complementation of myosin null mutants in Dictyostelium discoideum by direct functional selection”
  Develop. Biol. 137, 359-367. (Abstract) (pdf, 5.6 MB)
• Egelhoff, T.T., Brown, S.S., Manstein, D.J. & Spudich, J.A. (1989)
  “Hygromycin resistance as a selectable marker in Dictyostelium”
  Mol. Cell. Biol. 9, 1965-1968. (Abstract) (pdf,15 MB)
• Manstein, D.J., Ruppel, K.M. & Spudich, J.A. (1989)
  “Expression and cha­racterization of a functional myosin head fragment in Dictyostelium discoideum”
  Science 246, 656-658. (Abstract) (pdf, 5.6 MB)
• Manstein, D.J., Titus, M.A., De Lozanne, A. & Spudich, J.A. (1989)
  “Gene replacement in Dictyostelium: generation of myosin null mutants”
  (Abstract) EMBO J. 8, 923-932.
• Manstein, D.J., Massey, V., Ghisla, S. & Pai, E.F., (1988)
  “Stereochemistry and accessibility of prosthetic groups in flavoproteins”
  Biochemistry 27, 2300-2305. (Abstract) (pdf, 17 MB)
• Manstein, D.J., Pai, E.F., Schopfer, L.M., & Massey, V. (1986)
  “Absolute stereochemistry of flavins in enzyme catalyzed reactions”
  Biochemistry 25, 6807-6816. (Abstract) (pdf, 33 MB)
• Manstein, D.J. & Pai, E.F. (1986)
  “Purification and characterization of FAD synthetase from Brevibacterium ammoniagenes”
  J. Biol. Chem. 261, 16169-16973. (Abstract) (pdf, 9 MB)
• Manstein, D.J., Berriman, J., Leonard, K. & Rosenbusch, J.P. (1986)
  “Ultrastructure of native lipoprotein from Escherichia coli envelopes”
  J. Mol. Biol. 189, 701-707. (Abstract) (pdf, 14 MB)

Book Chapters

• Kazuhiro Oiwa and Dietmar J. Manstein (2007)
  “How linear motor proteins work”
  in Lecture Notes in Physics, 711, Controlled Nanoscale Motion – Nobel Symposium 131, H. Linke, A. Mansson (eds.) Springer, Berlin, Heidelberg, New York, pp. 41-59. (pdf, 880 kB)
• Manstein, D.J. (1993)
  “Myosin function in the motile behaviour of cells”
  in Cell Behaviour: Adhesion & Motility, Jones, G., Wigley, C., Warn, R. (eds.), The company of Biologists Limited, Cambridge UK, pp. 375-381. (pdf, 11 MB)
• Schröder, R.R., Manstein, D., Jahn, W., & Spudich, J.A. (1993)
  “Energy filtering and correction for the contrast transfer function (CTF) of frozen hydrated objects as illustrated by a reconstruction of Dictyostelium S1 decorated Actin”
  in Proc. 51st Annual Meeting of the Microscopy Society of America; Bailey, G.W. and Rieder C.L. (eds.), MSA, San Francisco Press, San Francisco, CA, pp. 118-119.
• Manstein, D.J., Massey, V. & Pai E.F. (1987)
  “Absolute stereochemistry of flavins”
  in Flavins & Flavoproteins, Edmondson, D.E., McCormick, D.B.(eds.) Walter de Gruyter & Co., Berlin, New York, pp. 3-12.
• Manstein, D.J. & Pai, E.F. (1987)
  “Purification and characterization of the bifunctional enzyme FAD synthetase from Brevibacterium ammoniagenes”
  in Flavins & Flavoproteins, Edmondson, D.E., McCormick, D.B.(eds.) Walter de Gruyter & Co., Berlin, New York, pp. 455-458.